Depends on what do you really want, the genes table represents the global expression of the gene (as a summary of all isoforms for the gene), the isoforms table is for each transcript of the gene. So, if you run DESeq2 in genes, you get genes, and isoforms, you get transcripts.
From a biological point of view, genes are representing a general function, the isoforms a more controlled regulation, if the biological response is to shut down the gene, you can expect all isoforms will be downregulated, but if the biological response requires to shut down a particular isoform, the gene will be not DE, but one isoform will be.
11 months ago by
JC ♦ 11k