Hello helpers, I just got raw reads from 10Xgenomics and started to do trimming and assembly. I’m new to this technology and have some basic questions below. Basically, I want to use software trim galore for trimming first and have clarify these first so as to choose proper options.
1, I only have one library. In the fastq file, what is the first line mean @K00162:247:HNJVCBBXX:5:1101:1357:1314 1:N:0:NCTCGTTT ; how to tell if the sequences are from different samples? I guess if they are from the same species, I don’t need to do any demultiplex?
2, Is the adapter sequences used in 10Xgenomics the same as illumina? 3, Is 10Xgenomics using ASCII+33 quality scores as Phred scores?
Thanks a lot for your help! Nunu