I have two lists of sequences and I need to calculate the similarity for every pair from these two lists. So I apply 'needle' from EMBOSS package. Everything goes fine until this sequence comes across: 'IDMSAYPVESIR'. When it happens the program crashes with the message:
Warning: Sequence 'embl::ref.txt:EMBOSS_001' has zero length, ignored Error: Unable to read sequence 'seq1.txt' Died: needle terminated: Bad value for '-asequence' and no prompt
Can anybody tell me, why is it happening? What may cause the problem ?