Question: miRWalk and Cytoscape to show microRNA-mRNA interactions
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gravatar for Shicheng Guo
13 months ago by
Shicheng Guo8.5k
Shicheng Guo8.5k wrote:

Hi All,

I have about 5 miRNAs and each of them I have some confirmed mRNA target, I want to use the following figure to show the network among these miRNA and mRNA. Is there any protocol?

Thanks.

enter image description here

ADD COMMENTlink written 13 months ago by Shicheng Guo8.5k
1

Dear @Shicheng Guo

Do you already have mapped the targets?

If so, create a txt file with the miRNA-target and import into cytoscape.

After that you will need to read a little in cytoscape's manual and use your creativity.

You can also see a brief explanation of how to deal with cytoscape here:

A: Cytoscape network of upregulate and downregulate transcription factors: how to d

If you have not mapped the targets....

You can get some insights here:

C: MicroRNA-target interactions databases

ADD REPLYlink modified 13 months ago • written 13 months ago by Leite1.1k

Thanks Leite, Your suggestion is really helpful!!

ADD REPLYlink written 12 months ago by Shicheng Guo8.5k
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