I have installed LAST tool to align nanopore reads. lastdb option is used to create database from fasta file for alignment. For metagenomic study, I need to align my nanopore reads to the NCBI nt (nucleotide) or nr (protein) database. How can I make a database for alignment using LAST tool from (NCBI nt or nr database) which is not in fasta format? Please help.
Can you give us some more insight in what exactly you try to achieve? It could be that LAST might not be the appropriate tool for it.
I want to align the nucleotide bases to the database for classification of microbes present.