Question: Identifying very closely related proteins
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gravatar for Baby Bioinformatician
11 months ago by
Moscow
Baby Bioinformatician90 wrote:

Hello Biostars, I have four dehydrogenases that exhibit stereospecificity for a compound. I am trying to identify the orthologs of these proteins in different genomes of streptomyces bacteria. However, since the four protein sequences are highly identical to each other, I get the same best hit for each of them when I perform a blast search in each genome. How best should I identify the orthologs of these proteins from my target genomes? Thank you for your suggestions.

ADD COMMENTlink modified 11 months ago by Mensur Dlakic6.9k • written 11 months ago by Baby Bioinformatician90
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gravatar for Mensur Dlakic
11 months ago by
Mensur Dlakic6.9k
USA
Mensur Dlakic6.9k wrote:

If your proteins are highly identical to each other - what is the exact identity level? - their orthologs are highly identical as well. Therefore, it is not surprising that all of them have the same top hit. Sequence comparisons that detect orthology are not meant for detecting these fine differences between related proteins such as stereospecificity.

Though I doubt that this can be done properly by sequence comparisons, here is an idea: if you know the protein region that confers stereospecificity, rank your BLAST matches by identity/similarity in that region rather than total scores.

ADD COMMENTlink written 11 months ago by Mensur Dlakic6.9k
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