Converting REFSeq IDs to Entrez IDs for KEGG analysis - "geneid of incorrect length"?
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23 months ago
saw44 ▴ 30

I have a list of REFSeq IDs from RNA seq and differential expression analysis. I want to run this through KEGG so I can see which pathways are up or down regulated.

For kegga() in R I need to convert these to Entrez IDs. I've used Biomart, but the output that I get has a different number of IDs to my original dataset. So, when I try and run kegga(), I get this error:

Error in kegga.MArrayLM(data, species = "Mm", geneid = gene_list\$ID) :
geneid of incorrect length


Is this because there are non-coding RNAs in my REFSeq dataset? How can I fix this problem so I can do my pathway analysis?

R rna-seq kegg deseq edger • 732 views
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Can you provide more information? Is there any missing value in the ID column?