Help Understanding bamtobed Flag -split
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Entering edit mode
4.4 years ago
Viz ▴ 50

Hello, as the title states I just need a little help fully understanding what the -split flag does in the bedtools utility, bamtobed. It states,

-split Report each portion of a “split” BAM (i.e., having an “N” CIGAR operation) alignment as a distinct BED intervals.

However, upon being asked about how this affects output I couldn't properly answer(It gives us expected results when used, when it is missing the results are wrong). I was wondering if someone could help me understand what exactly happens with this flag.

Link to bamtobed manual

rna-seq sequence • 1.0k views
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3
Entering edit mode
4.4 years ago

if you have a cigar string like this: 10M100N20M : the tool will break your record in two lines; one bed line of 10 bases (10M) and another one of 20 bases (20M), ignoring the 'intron' of 100 bp

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That's what I assumed but I just wanted to be sure, thank you so much! Accepted.

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