I'm reading up on the Seurat user guide: https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html And they mention for QC utilizing
The number of unique genes detected in each cell and The total number of molecules detected within a cell
They then refer to them as nCount_RNA and nFeature_RNA, but I'm not sure which is which. So my question is:
1.) What are the nCount_RNA and what are the nFeature_FNA 2.) Later in the pipeline, when you're normalizing the data, it says they "normalizes the feature expression measurements for each cell by the total expression." Can anybody explain that?