Question: region amplified by primers designed for bisulfite
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gravatar for rezaee.genetic
9 weeks ago by
rezaee.genetic0 wrote:

hi in many article i find the seq of primers designed for bisulfite sequencing, but i can't find the region that is amplified by those primers since they are compatible with changed cytosine to uracil. can you tell me a way I can find the seq that is amplified?

sequencing alignment gene • 91 views
ADD COMMENTlink modified 9 weeks ago by Pierre Lindenbaum125k • written 9 weeks ago by rezaee.genetic0

What exactly is the problem. Please give representative examples.

ADD REPLYlink written 9 weeks ago by ATpoint28k

for example in an article the promoter of oct4 gene has been studied for methylation through bisulfite sequencing.in the article the seq of the primers are available but if i put these primers in the ucsc in silico pcr no region will be amplified because the seq of primer has been designed supposing that unmethylated cytosines have been converted to uracil. my question is do you know a way I can find the region that is amplified by these primers in genome?

ADD REPLYlink written 8 weeks ago by rezaee.genetic0
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