I am running DEsingle (https://pubmed.ncbi.nlm.nih.gov/29688277-desingle-for-detecting-three-types-of-differential-expression-in-single-cell-rna-seq-data/) to compare two clusters of cells identified by Smart-seq2 scRNA-sequencing. I have filtered the genes before running DEsingle to retain only genes expressed in at least 10% of cells from one of the two clusters (5379 genes retained). DEsingle outputs the results but I get the following warning message. How concerned should I be about this message and should I trust the results?
Warning message: In MLE(ll3, start = listeta.mu = eta.mu, eta.sigma = eta.sigma, : possible convergence problem: optim gave code=1 function evaluation limit reached without convergence (9)