Question: Mega X help - phylogenetic tree
gravatar for SILE1_17
4 months ago by
SILE1_170 wrote:


I'm VERY new to bioinformatics, and for my uni course I'm doing a project analysing the geographic spread of chytrid fungus. I have used NCBI to identify Batrachochytrium dendrobatidis (chytrid fungus) ITS1 & ITS2 partial sequences, I have saved these in a FASTA file and uploaded them to Mega x to produce a phylogenetic tree. However my out-groups are only out when I use UPGMA, the bootstrap values are mostly very bad and the tree doesn't look right at all, so I think I must be doing something wrong or maybe my sequences are bad. Just looking for some help and advice.

Thank you!!!

ADD COMMENTlink written 4 months ago by SILE1_170

Is it possible to provide a picture of the tree? Did you look at the genetic differentiation of the sequences between samples? Would you expect good bootstrap values or a good resolution of the tree based on the genetic differentiation of the samples at all?

ADD REPLYlink modified 3 months ago • written 3 months ago by solanum10
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1637 users visited in the last hour