Question: Mega X help - phylogenetic tree
0
gravatar for SILE1_17
4 days ago by
SILE1_170
SILE1_170 wrote:

Hi,

I'm VERY new to bioinformatics, and for my uni course I'm doing a project analysing the geographic spread of chytrid fungus. I have used NCBI to identify Batrachochytrium dendrobatidis (chytrid fungus) ITS1 & ITS2 partial sequences, I have saved these in a FASTA file and uploaded them to Mega x to produce a phylogenetic tree. However my out-groups are only out when I use UPGMA, the bootstrap values are mostly very bad and the tree doesn't look right at all, so I think I must be doing something wrong or maybe my sequences are bad. Just looking for some help and advice.

Thank you!!!

ADD COMMENTlink written 4 days ago by SILE1_170

Is it possible to provide a picture of the tree? Did you look at the genetic differentiation of the sequences between samples? Would you expect good bootstrap values or a good resolution of the tree based on the genetic differentiation of the samples at all?

ADD REPLYlink modified 3 days ago • written 3 days ago by solanum10
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1104 users visited in the last hour