Hello! I have a dataset of 1092 human non-homologous proteins of Streptococcus pyogenes MGAS5005 (those are selected after following different subtraction processes) of which I want to find out the essential genes. Previously I have done this job using BLAST (with my dataset) against Database of essential genes (DEG). But DEG server is currently down and I badly need to do this job. Can anyone help me with any alternative option or simply how this work can be done without DEG?
Are you using this link : http://tubic.org/deg_bak/ ?
It seems that this database has moved here : http://www.essentialgene.org/
The following message appears on their website,
The new release is still under test. The old version is at: http://tubic.org/deg_bak/ The Genome-BLAST searches against DEG are available now. If you have any problems, please send email to Hao Luo.