Question: differential expression analysis with z score normalized microarray data
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gravatar for gracie
10 months ago by
gracie20
gracie20 wrote:

Hi all,

I am dealing with a Z normalized expression dataset and I want to perform differential expression analysis with two factor . I tried limma with two factor design (and also single) but couldnt find any gene altered significantly.

I am not sure is it because I am violating any assumptions of limma. Is there any other way for such analysis or any suggestions for the normalization of custom made microarray?

Thanks in advance

limma deg microarray • 243 views
ADD COMMENTlink modified 10 months ago by Biostar ♦♦ 20 • written 10 months ago by gracie20

Why not simply feeding the raw data into the common microarray workflows?

ADD REPLYlink written 10 months ago by ATpoint40k
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