Question: Data set containing corresponding RNA-seq and RT-Qpcr data
gravatar for chtrgswm
13 months ago by
chtrgswm20 wrote:

Can anybody please direct me to a study where they have published RNA seq and RT-qPCR data of the same organism? There are experiments that have validated RNA-seq using RT-qPCR, but they published only fold changes and not the raw Ct values. My work requires both the raw read counts and raw ct values. The disease is irrelevant.

sequencing rt-qpcr rna-seq • 252 views
ADD COMMENTlink modified 13 months ago • written 13 months ago by chtrgswm20

There is this quite old comparison of the MAQC data which is regularly repeated (e.g. in the ABRF study), but the original paper contained assays like TaqMan, QuantiGene, and StaRT-PCR:

ADD REPLYlink modified 13 months ago • written 13 months ago by michael.ante3.6k
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