I'm attempting to perform differential expression analysis using the
FindAllMarkers function included in
Seurat. I'd like to use the
DESeq2 function as I've had good results on bulk RNAseq using that package in the past. However, when I try to run it, it throws an error since there are so many zero values in my counts matrix. Would it be statistical malpractice to simply add 1 to each matrix entry? I'm pretty sure this is done in differential expression analysis of bulk RNAseq but it's been a while so I can't remember.