tblastn genome co-ordinate
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4.3 years ago
mdfardin374 ▴ 10

Hello everyone, I am trying to do tBLASTn for 500 peptide sequence derived from 6 frame translation of genome to get their genomic co-ordinate. Please tell me what cut off e-value should I keep so that every peptide in the database get hit. Should I also change the value of word size? Thank you

alignment genome • 507 views
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