Context and environment:
Institut Curie is one of the biggest European research institutions with strong and interdisciplinary traditions. It also comprises a hospital specialized in cancer treatment, and therefore disposes of a continuum of expertise from basic research to patient care.
It is located in the center of Paris in France, in a both cultural and scientific rich environment. Our immunology department includes 9 independent research teams in the fields of basic and applied immunology, working in a very collaborative and international environment.
Project:
The objective is to perform bioinformatics analyses on data produced with in vivo mice models and in vitro human models, to further improve our understanding of the fundamental mechanisms of immunology in areas such as: adaptive immunity, innate immunity, cancer immunotherapy, antiviral immunity (mainly HIV), dendritic cell biology and migration.
In close collaborations with other bioinformaticians, wet-lab postdocs and students, the selected candidate will design experiments, propose analytical approaches and analyze results obtained by NGS technologies:
- ChIP-Seq, RNA-Seq, Affymetrix, single-cell RNA-Seq, single-cell ChIP-seq
- Downstream functional analyses
The bioinformatician will work sequentially on diverse projects from 4 teams located on the same floor. He/She will benefit from the computing infrastructure of Institut Curie and from direct interactions with a core of bioinformaticians in our Immunology & Cancer department (10 people), with the Bioinformatics research department, with the Bioinformatics platform at Institut Curie and with the NGS platform.
Profile:
- Master or PhD in bioinformatics, biostatistics or in biology with experience in bioinformatics
- Experience in manipulation, analysis, and visualization of NGS data
- Former experience in the analysis of single-cell RNA-Seq data is desirable
- Knowledge of statistics tools (R, Bioconductor)
- Proficiency in at least one scripting language (python, perl, bash scripting)
- Experience in working on LINUX environments
- Knowledge of the human and/or mouse genome structure
- Ability to communicate in an interdisciplinary research group
- Experience in working on HPC environments would be a plus
Duration: 3-year contract, salary depends on profile and experience. The contract can be renewed based on success. Long-term perspectives are available.
This position is highly competitive and only outstanding candidates will be considered. Please send CV and two letters of reference to nicolas.manel@curie.fr
References:
- Johnson J, Lahaye X, Manel N, Ménager M et al. A comprehensive map of the dendritic cell transcriptional network engaged upon innate sensing of HIV. Cell Reports, in press. doi: 10.1101/579920
- N Ruffin ... P Benaroch. Constitutive Siglec-1 expression confers susceptibility to HIV-1 infection of human dendritic cell precursors. Proc Natl Acad Sci U S A. 2019. doi: 10.1073/pnas.1911007116
- Lahaye X ... Manel N. NONO Detects the Nuclear HIV Capsid to Promote cGAS-Mediated Innate Immune Activation. Cell. 2018. doi:10.1016/j.cell.2018.08.062
- Silvin A ... Manel N. Constitutive resistance to viral infection in human CD141(+) dendritic cells. Science Immunology. 2017. doi:10.1126/sciimmunol.aai8071
- Decalf, J ...Benaroch, P. Sensing of HIV-1 entry triggers a type I interferon response in human primary macrophages. J Virol. 2017. doi:10.1128/JVI.00147-17.
- Saitakis M, ..., Hivroz C. Different TCR-induced T lymphocyte responsesa re potentiated by stiffness with variable sensitivity. Elife. 2017. doi:10.7554/eLife.23190.
- Gentili M ... Manel N. Transmission of innate immune signaling by packaging of cGAMP in viral particles. Science. 2015. doi: 10.1126/science.aab3628
