I have calculated
Variant Allele Frequency (VAF) for called SNVs and INDELs called by Strelka separately. For getting VAF, I done
VAF = Tumour Variant Allele Count / Tumour Read Count
For some position of the genome I have
VAF > 30 , so DOES these big VAFs are normal or I am doing something wrong?
I was supposing VAFs should be in the range of
0 < VAF <1
Can you help in getting some idea?