I have treatment and control samples single cell sequencing performed using 10x method. I wanted to know how to calculate the differential expression of genes between two groups. I am trying to use this tutorial from seurat but it does not say how to import two different sample dataset into seurat. Can someone recommend easier way to perform this analysis in single cell experiments.
Taking the time out to follow the Seurat tutorial is worth it. Single cell data have so many biases and problems you will need to have under control for your differential expression analysis to be trustworthy.
With regards to your data I would recommend using Seurat3's data integration to create a joint dataset - a nice tutorial equivalent to your data can be found here. Afterwards you can follow the tutorial you refere to above. An alternative would be CONOS - you can find that tutorial here. Furthermore if you have multiple replicates for your data you could consider muscat.