Question: (Closed) Plasmid sequencing through illumina Miseq
0
gravatar for asim
8 months ago by
asim0
south korea
asim0 wrote:

I am a newbie at plasmid sequencing. I want to sequence plasmids isolated from Vibrio through illumina Miseq 300bp PE platform. Whether i will get the complete plasmid and can i pool my all plasmids together for a single Miseq run to save money? Secondly can i get the complete plasmid sequence through 300bp PE Miseq and for each run of miseq how much it will cost? Thank You

sequencing • 362 views
ADD COMMENTlink modified 7 months ago by Biostar ♦♦ 20 • written 8 months ago by asim0

Hello asim!

We believe that this post does not fit the main topic of this site.

Not really boinformatics.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

ADD REPLYlink written 7 months ago by ATpoint38k

Just to sum this up:

1) Yes you can pool samples. The keyword here would be multiplexing via different indices. Please google for more information on that. The library prep kits cover that.

2) For pricing please visit the Illumina website or get a quote from a sequencing provider. MiSeq v3 300cycles is somewhat 1000€.

ADD REPLYlink written 7 months ago by ATpoint38k
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