How to use SingleR for annotating single-cell transcriptomics dataset with our own custom made bulk RNA data?
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15 months ago
A248 ▴ 10

Is this possible on the latest SingleR release version (3.10)? It looks like its fairly easy to use pre-existing Bulk RNA signatures from the built-in references, but when the bulk RNA data is generated by ourselves, is this possible?

Thanks in advance!

single cell sequencing annotation singleR • 346 views
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15 months ago

Sure, you just have to feed it the counts (ref argument) and a vector of labels (labels argument) corresponding to each sample. Reading ?SingleR should explain pretty clearly.

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