Off topic:how to get low depth variants in vcf file (hetrozygous condition)
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4.3 years ago

I want to get a variant in vcf file which is present in hetrozygous condition. for example if at a specific position 50 reads get mapped and 26 of them are same as reference and 24 reads have an alternate allele. that specific position didn't show up as variant in vcf file. But I want them in vcf file as they are very important to me. How can I do that ???

SNP next-gen alignment • 427 views
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