Question: Aspera NCBI upload: errors and permissions?
0
gravatar for Lumengmeng_bunny
10 months ago by
Lumengmeng_bunny0 wrote:

I'm trying to upload about 30GB metagenomic data to NCBI SRA database using Aspera Connect. I used the code recommended and when some questions arose, I think I found ways to solve them and then I thought I did it. Then some errors appeared,when I print:

cd C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin\
C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin> ascp -i C:/Users/MM/Desktop/aspera.openssh/-QT -l100m -k1 -d C:/Users/MM/Documents/OriginalData/AME_1.fastqsubasp@upload.ncbi.nlm.nih.gov:uploads/congren_jiangnan.edu.cn_0JDwGYDO

errors as below :

ascp.exe: no remote host specified
Startup failed, exit

Has anybody faced such a problem? What should I do to solve it? What does "no remote host specified" mean?

software error • 685 views
ADD COMMENTlink modified 10 months ago by genomax92k • written 10 months ago by Lumengmeng_bunny0
3
gravatar for genomax
10 months ago by
genomax92k
United States
genomax92k wrote:

This may be just a copy paste error but make sure there is a space between your fastq file name and the server name. There does not seem to be at the moment. Try this:

C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin> ascp -i C:/Users/MM/Desktop/aspera.openssh/-QT -l100m -k1 -d C:/Users/MM/Documents/OriginalData/AME_1.fastq subasp@upload.ncbi.nlm.nih.gov:uploads/congren_jiangnan.edu.cn_0JDwGYDO
ADD COMMENTlink modified 10 months ago • written 10 months ago by genomax92k

Thank you. But another error arose... "Error: 绯荤粺鎵句笉鍒版寚瀹氱殑鏂囦欢銆?" What does this mean?

ADD REPLYlink written 10 months ago by Lumengmeng_bunny0

Since I don't understand chinese this error likely has to do with the language being used (based on searching the term above). Aspera expects to see English is my best guess.

ADD REPLYlink written 10 months ago by genomax92k

Anyway, thank you very much

ADD REPLYlink written 10 months ago by Lumengmeng_bunny0
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