Aspera NCBI upload: errors and permissions?

Question

[Lumengmeng_bunny]

I'm trying to upload about 30GB metagenomic data to NCBI SRA database using Aspera Connect. I used the code recommended and when some questions arose, I think I found ways to solve them and then I thought I did it. Then some errors appeared,when I print:

cd C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin\
C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin> ascp -i C:/Users/MM/Desktop/aspera.openssh/-QT -l100m -k1 -d C:/Users/MM/Documents/OriginalData/[e-mail]:uploads/congren_jiangnan.edu.cn_0JDwGYDO

errors as below :

ascp.exe: no remote host specified
Startup failed, exit

Has anybody faced such a problem? What should I do to solve it? What does "no remote host specified" mean?

Answer 1

[GenoMax]

This may be just a copy paste error but make sure there is a space between your fastq file name and the server name. There does not seem to be at the moment. Try this:

C:\Users\XX\AppData\Local\Programs\Aspera\Aspera Connect\bin> ascp -i C:/Users/MM/Desktop/aspera.openssh/-QT -l100m -k1 -d C:/Users/MM/Documents/OriginalData/AME_1.fastq [e-mail]:uploads/congren_jiangnan.edu.cn_0JDwGYDO

[Lumengmeng_bunny]

Thank you. But another error arose... "Error: 绯荤粺鎵句笉鍒版寚瀹氱殑鏂囦欢銆?" What does this mean?

[GenoMax]

Since I don't understand chinese this error likely has to do with the language being used (based on searching the term above). Aspera expects to see English is my best guess.

[Lumengmeng_bunny]

Anyway, thank you very much