Hi Everyone, I am very new to scRNA-seq analysis, and I'm trying to analyze some scRNA-seq data. The goal of the analysis is to compare lncRNA and mRNA genes by calculating:- overall mean expression of lncRNA genes and mRNA genes - and expression levels f lncRNA genes and mRNA genes.
So far, I performed the following on my data: QC analysis, normalization, identification of differentially expressed genes, scaling and regressing out some housekeeping gens - all done using the Seurat pipeline in R.
Please I do not know what do do next from here and seem to be stuck on what to do. Also if anyone once could recommend a good easy to follow tutorial on how to go about this, it would be appreciated.
Any feedback would be most appreciated. Thanks.