Question: Paired End RIP-seq analysis with MACS2 reasonable parameters?
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gravatar for ddierks91
11 weeks ago by
ddierks910
ddierks910 wrote:

Since some recent publications involving MACS2 as a valuable RIP-Seq (RNA not DNA) peak caller. I tried it to use on my data. But I realize that it since its a Chip-Seq peak-caller, has issues with the paired-end calculated insert/fragment sizes. I work with poly-A selected Material (mostly mRNA lacking Introns).

So I wonder if there are any parameters where I can use actual Fragment Sizes not in a genomic setting (but based on transcript annotations)?.

Otherwise, I don't see how MACS2 can be a reasonable paired-end Peak Caller for RIP-seq analysis.

Greetings and Thanks! David

ADD COMMENTlink written 11 weeks ago by ddierks910

What do you mean with has issues with the paired-end calculated insert/fragment sizes.? It has a -f BAMPE option that simply reads TLEN. Please explain.

ADD REPLYlink written 10 weeks ago by ATpoint32k
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