I am planning to re-analyze the scRNA-seq data on SRA (SRR9713131, https://www.ncbi.nlm.nih.gov/sra/?term=SRR9713131). According to the description of this data it is pair-end data and the first end contain sequence of UMI. However, I found the sequence of this data is single end that in “Reads” sheet of “Run Browser” page. I downloaded it (sra-format file) and converted it into fastq-format using SRAtoolkit and confirmed it indeed is single-end.
My questions are below:
Is it really a single-end data instead of pair-end data? Did I make some mistake?
How and why does this data pass the quality check if it is single-end, since single-end of scRNA-seq is no use?