refRANK error: Assembly_genomic.fna contains multiple FASTA entries
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15 months ago

Hi

I'm using refRANK to determine the most suitable reference genome to use for downstream analysis on some fastq STEC sequences. I downloaded around 70 STEC genomes from NCBI, transferred them to HPC and unzipped the tar file so they are now in genomic.fna format. However, each time I run the script its returning an error saying Assembly_genomic.fna contains multiple FASTA entries. Anyone have any ideas on how to solve this? Any advice would be much appreciated. The script is outlined below.

module load python3.7/i2

python refrank.py --fastq *.fastq.gz --ref *.fna

software error • 241 views
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