Question: predicted protein and sequence
0
gravatar for mdfardin374
8 months ago by
mdfardin37410
mdfardin37410 wrote:

Hello all, I have a peptide of 20 aa. When I map this peptide to the genome, the first 12 aa map to exon1 in frame 1 and last 8 aa map to exon2 in frame 3. Is it possible?

alignment genome • 151 views
ADD COMMENTlink written 8 months ago by mdfardin37410

How confident are you about the genome sequence? It could be incorrect. In addition to the possibility that you have wrong peptide sequence.

ADD REPLYlink written 8 months ago by genomax91k

The transcriptome was translated into three frames and the obtained peptide was MS/MS analyzed. The genome sequence is correct.

ADD REPLYlink written 8 months ago by mdfardin37410
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