Question: predicted protein and sequence
0
gravatar for mdfardin374
12 months ago by
mdfardin37410
mdfardin37410 wrote:

Hello all, I have a peptide of 20 aa. When I map this peptide to the genome, the first 12 aa map to exon1 in frame 1 and last 8 aa map to exon2 in frame 3. Is it possible?

alignment genome • 188 views
ADD COMMENTlink written 12 months ago by mdfardin37410

How confident are you about the genome sequence? It could be incorrect. In addition to the possibility that you have wrong peptide sequence.

ADD REPLYlink written 12 months ago by GenoMax96k

The transcriptome was translated into three frames and the obtained peptide was MS/MS analyzed. The genome sequence is correct.

ADD REPLYlink written 12 months ago by mdfardin37410
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