Question: PTC positions in transcripts
gravatar for jsw940
7 months ago by
jsw94010 wrote:

Hello, I have a question about Ensembl genome data.

There are lots of transcripts annotated as "nonsense_mediated_decay" in ensembl data.

Then, I think that PTC(premature termination codon) must be in that transcript. So, how can I know the position and kinds of PTC which is the evidence of annotation?

I used to try to find PTCs based on ORF. But because there are number of cases, I couldn't find PTC.

How could I find it?

Thank you.

sequencing rna-seq • 149 views
ADD COMMENTlink modified 7 months ago by i.sudbery9.2k • written 7 months ago by jsw94010
gravatar for i.sudbery
7 months ago by
Sheffield, UK
i.sudbery9.2k wrote:

This depends on how you define a PTC (which in turn depends on how the cell detects a termination codon as a PTC). The usual theory is that a cell detects a stop codon as a premature stop codon if there are exon junctions downstream of the stop. Thus Ensembl will mark any gene which has an intron after the stop codon as "nonsense_mediated_decay", and the supposed "PTC" is the one at the end of the ORF.

ADD COMMENTlink written 7 months ago by i.sudbery9.2k
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