Hi, I've noticed that the Wilcoxon rank-sum test is used by many R packages to calculate differential expression. Is this a good test to estimate that when you compare it to other packages like DESeq2 and edgeR which calculate differential expression with many more factors involved and would that not be better than just a Wilcoxon test?

So do you mean that one should trust Wilcoxon if the number of cells in question is less (eg - <100) and if the number cells are higher (>100 or more), it is better to trust DESeq2 instead?