Entering edit mode
4.2 years ago
zhamouda
•
0
Hi I'm new to bioinformatics and I get an empty file everytime I am trying to filter variants with HapMap3 "HMR". In my VCF file this information is contained in INFO column like this:
INFO=<ID=HM3,Number=0,Type=Flag,Description="HapMap3 membership"
and header
#CHROM POS ID REF ALT QUAL FILTER INFO
1 1110294 rs1320571 G A . PASS AA=A;AC=67;AN=166;DP=5401;HM2;HM3
According to vcftools documentation there is option to filter sites with specific INFO flag (--keep-INFO). I've tried to use this:
vcftools --vcf file.vcf --out output --keep-INFO ID=HM3,Number=0,Type=Flag,Description="HapMap3 membership"
and the warning msg
VCFtools - 0.1.16
(C) Adam Auton and Anthony Marcketta 2009
Parameters as interpreted:
--vcf pilot.vcf
--out try
--keep-INFO ID=HM3,Number=0,Type=Flag,Description=HapMap3 membership
Warning: Expected at least 2 parts in INFO entry: ID=AA,Number=1,Type=String,Description="Ancestral Allele, ftp://ftp.1000genomes.ebi.ac.uk/vol1/ftp/pilot_data/technical/reference/ancestral_alignments/README">
After filtering, kept 0 out of 0 Individuals
Error: Using INFO flag filtering on non flag type ID=HM3,Number=0,Type=Flag,Description=HapMap3 membership will not work correctly.
how properly should I use this function?