Hi! Im new here. I started doing the RNA-Seq analysis. I have data from Illumina. I did the quality control with fastqc and tried to trim it using trimmomatic program and my data didnt change at all. I take under consideration that maybe i should change the values i used. Heres the command i used. I would appreciate every help.
java -jar /path../Trimmomatic-0.39/trimmomatic-0.39.jar PE UP1_1.fastq UP1_2.fastq UP1_trimmed_1.fq UP1_unpaired_1.fq UP1_trimmed_1.fq UP1_unpaired_2.fq ILLUMINACLIP:adapter.fa:2:30:5
Is it possible that i dont need to trim my data? Thanks for every respond :)