i have 40 SARCOMA data. and there are many groups of tissue.
if i use RNA expression normalization method, is it okay to compare?
More specific, if i have 12 bone tissue sarcoma and 28 Uterus tissue type.
using these 40 sample, the edgeR results in MDM4 gene is differentially expression in tumor than normal.
i think it is nonsense to compare.
am i right?