Question: location of integrated vector in the genome in CRISPR experiment
0
gravatar for Sara
12 months ago by
Sara160
Sara160 wrote:

I have done a CRISPR experiment followed by whole genome sequencing and now I am trying to find the position of integrated vector in the genomes. is there any tool for this goal?

genome • 222 views
ADD COMMENTlink modified 12 months ago by lieven.sterck10k • written 12 months ago by Sara160

what kind of data do you have?

your question is very brief, Please go through [[ Please read before posting a question ::: How To Ask A Good Question ]] and then consider editing your question.

ADD REPLYlink written 12 months ago by lieven.sterck10k

Adding on this, you will notice that many of your previous questions went unanswered, and I think the lack of effort and details is a major factor for this. Is this lentiviral? What exactly did integrate into the genome and how?

ADD REPLYlink written 12 months ago by ATpoint46k

@ATpoint: sorry. yes it is lentiviral

ADD REPLYlink written 12 months ago by Sara160

@lieven.sterck: I have done some edits. hope it is more informative.

ADD REPLYlink written 12 months ago by Sara160
0
gravatar for lieven.sterck
12 months ago by
lieven.sterck10k
VIB, Ghent, Belgium
lieven.sterck10k wrote:

The approach offered by Brian Bushnell in this post Identification of the sequence insertion site in the genome has proven its value and efficiency several times for me when identifying Tdna insertion sites (I'm assuming it would work as well for CRISPR insertions).

The only requisite is that you know the sequence that has been inserted.

ADD COMMENTlink written 12 months ago by lieven.sterck10k
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2349 users visited in the last hour
_