location of integrated vector in the genome in CRISPR experiment
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16 months ago
Sara ▴ 160

I have done a CRISPR experiment followed by whole genome sequencing and now I am trying to find the position of integrated vector in the genomes. is there any tool for this goal?

genome • 275 views
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what kind of data do you have?

your question is very brief, Please go through [[ Please read before posting a question ::: How To Ask A Good Question ]] and then consider editing your question.

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Adding on this, you will notice that many of your previous questions went unanswered, and I think the lack of effort and details is a major factor for this. Is this lentiviral? What exactly did integrate into the genome and how?

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@ATpoint: sorry. yes it is lentiviral

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@lieven.sterck: I have done some edits. hope it is more informative.

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16 months ago

The approach offered by Brian Bushnell in this post Identification of the sequence insertion site in the genome has proven its value and efficiency several times for me when identifying Tdna insertion sites (I'm assuming it would work as well for CRISPR insertions).

The only requisite is that you know the sequence that has been inserted.

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