Question: location of integrated vector in the genome in CRISPR experiment
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gravatar for Sara
9 months ago by
Sara150
Sara150 wrote:

I have done a CRISPR experiment followed by whole genome sequencing and now I am trying to find the position of integrated vector in the genomes. is there any tool for this goal?

genome • 191 views
ADD COMMENTlink modified 9 months ago by lieven.sterck9.1k • written 9 months ago by Sara150

what kind of data do you have?

your question is very brief, Please go through [[ Please read before posting a question ::: How To Ask A Good Question ]] and then consider editing your question.

ADD REPLYlink written 9 months ago by lieven.sterck9.1k

Adding on this, you will notice that many of your previous questions went unanswered, and I think the lack of effort and details is a major factor for this. Is this lentiviral? What exactly did integrate into the genome and how?

ADD REPLYlink written 9 months ago by ATpoint42k

@ATpoint: sorry. yes it is lentiviral

ADD REPLYlink written 9 months ago by Sara150

@lieven.sterck: I have done some edits. hope it is more informative.

ADD REPLYlink written 9 months ago by Sara150
0
gravatar for lieven.sterck
9 months ago by
lieven.sterck9.1k
VIB, Ghent, Belgium
lieven.sterck9.1k wrote:

The approach offered by Brian Bushnell in this post Identification of the sequence insertion site in the genome has proven its value and efficiency several times for me when identifying Tdna insertion sites (I'm assuming it would work as well for CRISPR insertions).

The only requisite is that you know the sequence that has been inserted.

ADD COMMENTlink written 9 months ago by lieven.sterck9.1k
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