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4.2 years ago
t4192
▴
20
I am very confused by the NCBI website BLAST report!
When I put a 45kb plasmid into NCBI website BLAST , I get the result which is that, each line in the report is a complete plasmid. So what do the "Max Score", "Total Score", "Query Cover", "E value", "Per. Ident" mean? Actually, 45kb plasmid has many alignments with a plasmid in one line in the report.
When I do the local computer blastn program, the result is clear, because each line in the report is only one alignment. The meaning of each line and column are clear.
So please guide me through the mix-up in the NCBI website BLAST report.
NCBI provides lots of learning resources for BLAST. Please take some time and go through them.
If you learn visually then see these videos:
Another question. How can I transfer the local computer blastn result into the website BLAST report format. Because I think the website BLAST report format is easy for me to choose the useful complete sequence. I don't know how to sort my local computer blastn result which will help me select the useful complete sequence.
What format are you storing your local blast results in? If you add the
-htmloption you would get something akin to what you see on NCBI web page. NCBI obviously does fancy post-processing of the results which is not fully replicable on a local machine.I found a youtube video, please watch the content after 3:00. Do you understand what the video explains?