Question: what is the definition of "cell barcode collapsing"
0
gravatar for yueli7
4 weeks ago by
yueli7110
China
yueli7110 wrote:

Hello,

What is the meaning of the following figure?

Summay plot of cell barcode collapsing.

Plot the original frequency of each cell barcode in the original whitelist against the frequency after collapsing similar cell barcodes.

Thanks in advance for any help!

Best,

Yue

Screenshot-from-2020-02-26-17-35-02

rna-seq • 104 views
ADD COMMENTlink modified 4 weeks ago by Devon Ryan94k • written 4 weeks ago by yueli7110
3
gravatar for Devon Ryan
4 weeks ago by
Devon Ryan94k
Freiburg, Germany
Devon Ryan94k wrote:

The barcode collapsing is happening when barcodes contain sequencing errors and are therefore being corrected and then added to the correct barcode. Just as a made up example, if a ACTGACTG is a known barcode and has 2000 counts and ACTGACTC is not a known barcode and has 3 counts then those 3 counts would added to those from ACTGACTG, since that was presumably the original (non-error-containing) cell barcode.

ADD COMMENTlink written 4 weeks ago by Devon Ryan94k

Hello, Devon Ryan,

Thank you so much for your response!

I understood.

Thank yo again!

Best,

Yue

ADD REPLYlink written 29 days ago by yueli7110
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