GATK genotypemergeoption Error
0
0
Entering edit mode
4.1 years ago

Hi all,

I am trying to run a single sample dataset of a paired-end sample obtained for exome sequencing (Read 1 and Read 2) through the seqmule pipeline and it gives this error:

NOTICE: normalzing VCF with Vt...
NOTICE: done normalzing.
NOTICE: keep variants if they are unfiltered in any one of the input files.
NOTICE: extracting variants shared by at least 2 input files.
##### ERROR ------------------------------------------------------------------------------------------
##### ERROR A USER ERROR has occurred (version 3.8-1-0-gf15c1c3ef): 
##### ERROR
##### ERROR This means that one or more arguments or inputs in your command are incorrect.
##### ERROR The error message below tells you what is the problem.
##### ERROR
##### ERROR If the problem is an invalid argument, please check the online documentation guide
##### ERROR (or rerun your command with --help) to view allowable command-line arguments for this tool.
##### ERROR
##### ERROR Visit our website and forum for extensive documentation and answers to 
##### ERROR commonly asked questions https://software.broadinstitute.org/gatk
##### ERROR
##### ERROR Please do NOT post this error to the GATK forum unless you have really tried to fix it yourself.
##### ERROR
##### ERROR MESSAGE: Duplicate sample names were discovered but no genotypemergeoption was supplied. To combine samples without merging, specify --genotypemergeoption UNIQUIFY. Merging duplicate samples without specified priority is unsupported, but can be achieved by specifying --genotypemergeoption UNSORTED.
##### ERROR ------------------------------------------------------------------------------------------
Failed to merge VCF files
NOTICE: Cleaning...

When I looked at "genotypemergeoption" everywhere (in all the files and directories), they all had UNSORTED provided in the command for "genotypemergeoption", so I do not understand why the error is telling me that "genotypemergeoption" was not supplied.

Any help will be very much appreciated! Thank you!

gatk seqmule • 901 views
ADD COMMENT

Login before adding your answer.

Traffic: 1867 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6