Question: How to covert RKPM into absolute quantification?
gravatar for marongiu.luigi
6 months ago by
Germany, Mannheim, UMM
marongiu.luigi520 wrote:

Hello, is it possible to convert RPKM values into absolute values? That is to convert a qualitative (relative) measurement into a quantitative (absolute) measurement? If I have an RPKM of, let's say, 375 for a gene of interest (or micro-organism) and the sample was 1 mL of something, can I calculate the number of initial copies of the gene?

sequencing alignment genome • 211 views
ADD COMMENTlink modified 6 months ago by Kevin Blighe65k • written 6 months ago by marongiu.luigi520

If you don't have a spike-in of known concentration is impossible. See answers to a similar question here

ADD REPLYlink written 6 months ago by Fabio Marroni2.6k
gravatar for Devon Ryan
6 months ago by
Devon Ryan96k
Freiburg, Germany
Devon Ryan96k wrote:

This is not possible with regular RNA-seq data.

ADD COMMENTlink written 6 months ago by Devon Ryan96k
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