Perform differential circular RNA expression
0
0
Entering edit mode
13 months ago
wangdp123 ▴ 250

Hi there,

I am using CIRCexplorer2 to annotate circRNAs from the paired-end RNA-Seq datasets and obtain a file named "circularRNA_known.txt" with the column of number of junction reads.

1) What is the cutoff to defined the expressed circRNAs according to the number of junction reads of each annotated circRNAs?

2) How to perform the differential analysis between two groups of samples based on the number of junction reads?

3) How to calculate the RPM (Reads Per Million mapped reads) based on the paired-end data as I have been aware that CIRCexplorer2 will convert paired-end reads into single reads before doing the analysis?

Many thanks,

Tom

circRNA CIRCexplorer2 • 441 views
ADD COMMENT

Login before adding your answer.

Traffic: 1184 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6