Blastn query file with multiple sequences?

Question

[biogirl13]

Hi, I am pretty new to using the blastn command on the command line, and I wanted a little help figuring things out. So I made my own blast database using the makeblastdb command, and now am using the blastn command. I have a fasta file with 3 different sequences in it. When I use the blastn command with this fasta file, does it blast all 3 sequences? I only want to blast one of the sequences in the fasta file, so what do I write in my command to specify which sequence in the file to use? Thanks!

Answer 1

[AsoInfo]

You can make a new file containing only that sequence and then BLAST against that db file.

[biogirl13]

Is there another way to do this without making a new file?