Entering edit mode
4.0 years ago
zekunmu95
•
0
Hi! I'm wondering why 10x Feature Barcoding uses a capture sequence instead of a poly(A) for the feature barcoding DNA?
Hi! I'm wondering why 10x Feature Barcoding uses a capture sequence instead of a poly(A) for the feature barcoding DNA?
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If you are using a CRISPR guide RNA then it would (or an antibody). Here is 10x help page on all things related to feature barcoding.
Thank you! I figured out that we're doing 5' so poly(A) cannot be used anyways.