Downloading FASTQ with aspera
1
1
Entering edit mode
14 months ago
dagurim ▴ 10

Hello everyone

I used to download FASTQ files form ENA server with aspera, but I cant download datas anymore.

This code is what I used to use. (Thanks to SRA explorer)

ascp -QT -l 300m -P33001 -i $HOME/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@fasp.sra.ebi.ac.uk:vol1/fastq/SRR581/008/SRR5817038/SRR5817038_2.fastq.gz . && mv SRR5817038_2.fastq.gz SRR5817038_GSM2699772_8858-3_Hepa_Day_130_bulk_Homo_sapiens_RNA-Seq_2.fastq.gz

after some times, there was no download start signal nor error log. just new command line starts.

I tried to eliminate -P33001 parameter then it shows

ascp: Failed to open TCP connection for SSH, exiting.

Session Stop  (Error: Failed to open TCP connection for SSH)

I allowed port 22 and port 33001 with ufw long time ago.

I changed my internet to figure out whats going on, but it doesn`t work.

Is there any download policy change from ENA server?

Thank you very much.

sra • 1.3k views
ADD COMMENT
1
Entering edit mode

It is possible that aspera is down as someone else was having trouble with it yesterday. Try plain http/ftp links for now.

ADD REPLY
0
Entering edit mode

Tyvm.

Seems like ENA sever issue.

ADD REPLY
0
Entering edit mode

Yes. Few days ago ENA posted about they going to upgrade and it may affect data download. It may take 7-10 days to fully restore.

ADD REPLY
0
Entering edit mode
13 months ago
ATpoint 49k

Currently now not (sorry for the typo) possible, see Ensembl reduced functionality: 10th March-4th April 2020

ADD COMMENT
0
Entering edit mode

looks like ascp sever is still down. You can find the comment about it too. Are you able to download via ascp? Thank you

ADD REPLY
0
Entering edit mode

@ATPoint should have said NOT possible.

ADD REPLY
1
Entering edit mode

ascp now working for me as of Apr 9th!

ADD REPLY

Login before adding your answer.

Traffic: 2083 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6