Downloading FASTQ with aspera
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4.1 years ago
dagurim ▴ 10

Hello everyone

I used to download FASTQ files form ENA server with aspera, but I cant download datas anymore.

This code is what I used to use. (Thanks to SRA explorer)

ascp -QT -l 300m -P33001 -i $HOME/.aspera/connect/etc/asperaweb_id_dsa.openssh era-fasp@fasp.sra.ebi.ac.uk:vol1/fastq/SRR581/008/SRR5817038/SRR5817038_2.fastq.gz . && mv SRR5817038_2.fastq.gz SRR5817038_GSM2699772_8858-3_Hepa_Day_130_bulk_Homo_sapiens_RNA-Seq_2.fastq.gz

after some times, there was no download start signal nor error log. just new command line starts.

I tried to eliminate -P33001 parameter then it shows

ascp: Failed to open TCP connection for SSH, exiting.

Session Stop  (Error: Failed to open TCP connection for SSH)

I allowed port 22 and port 33001 with ufw long time ago.

I changed my internet to figure out whats going on, but it doesn`t work.

Is there any download policy change from ENA server?

Thank you very much.

sra • 2.9k views
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1
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It is possible that aspera is down as someone else was having trouble with it yesterday. Try plain http/ftp links for now.

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0
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Tyvm.

Seems like ENA sever issue.

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Yes. Few days ago ENA posted about they going to upgrade and it may affect data download. It may take 7-10 days to fully restore.

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4.1 years ago
ATpoint 81k

Currently now not (sorry for the typo) possible, see Ensembl reduced functionality: 10th March-4th April 2020

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looks like ascp sever is still down. You can find the comment about it too. Are you able to download via ascp? Thank you

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@ATPoint should have said NOT possible.

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ascp now working for me as of Apr 9th!

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