How to analyze DNA data with UMI
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4.3 years ago

Hi all!

I'm facing with analyze DNA exomes sequencing data with UMI. From the demultiplexing, I have three fastq files: one for reads R1, one for the UMI tags, and one for the R2. Now, I have to align data to the reference genome and call variants. Have you some suggestions for me?

Does anyone know some "best practices" for that?

Thanks
Denise

next-gen-sequencing umi • 1.6k views
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Have you looked at documentation of standard software such as UMI-tools?

Also, see How to take in consideration UMI in WES pipeline?. By the way, Ian (the guy who answers this question) is the author of UMI-tools.

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