Question: How to analyze DNA data with UMI
gravatar for deniselavezzari
10 months ago by
deniselavezzari0 wrote:

Hi all! I'm facing with analyze DNA exomes sequencing data with UMI. From the demultiplexing, I have three fastq files: one for reads R1, one for the UMI tags, and one for the R2. Now, I have to align data to the reference genome and call variants. Have you some suggestions for me? Does anyone know some "best practices" for that?

Thanks Denise

ADD COMMENTlink modified 10 months ago by GenoMax95k • written 10 months ago by deniselavezzari0

Have you looked at documentation of standard software such as UMI-tools?

Also, see How to take in consideration UMI in WES pipeline?. By the way, Ian (the guy who answers this question) is the author of UMI-tools.

ADD REPLYlink modified 10 months ago • written 10 months ago by ATpoint44k
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