Question: Demultiplex PacBio CCS reads on Mac
gravatar for manthony5955
11 months ago by
manthony59550 wrote:

I have CCS reads from a PacBio Sequel platform (.bam, .fastq, .fasta files). My library was multiplexed. The same barcode (12 bp) is attached to both sides of the sequence (symmetric). To demultiplex, I know of Lima, but this only appears to work for linux. I have a Mac and would like to do everything on it (if possible).

Any help would be great. I suspect there may be a bamtools solution.

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