how to annotate cluster file which is not .bed format
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4.0 years ago
xiaoleiusc ▴ 140

Dear All,

I run Paralyzer to process my PAR-CLIP data and the output cluster file is a .csv file which looks like below (or please see screenshot https://drive.google.com/file/d/1UhcJXHNYavKDA7LdusYaV7QTbN8g1kG-/view?usp=sharing): Chromosome Strand ClusterStart ClusterEnd ClusterID ClusterSequence ReadCount ModeLocation ModeScore ConversionLocationCount ConversionEventCount NonConversionEventCount 11 + 109473923 109473958 G684.1 GGCCGTGATCGTATAGTGGTTAGTACTCTGTGTTGT 7692 109473953 0.700221808 14 13927 86395 16 + 33963662 33963764 G1400.1 GGTTCCGGGGGGAGTACGGTTGCAAAGCTGAGTTA 7188 33963715 0.999418794 11 319 26443

I wonder how I could annotate this cluster file with hg19 GTF as an annotation file? The content in this file is not a bed format, and contains many columns with information specifically related to Paralyzer.

Thanks ahead for any input.

Sincerely,

Xiao

RNA-seq; CLIP-seq • 490 views
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