I'm using MAST package to detect differentially expressed genes in single cell RNAseq datasets. I've used DESeq2 in the past and I know that it selects the reference group for calculating fold changes alphabetically if not pre-defined. I'm struggling to understand if MAST does the same thing? Does it select the reference group for FC calculation alphabetically? It's not very clear from the documentation available.
Thanks in advance for helping,