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I actually wanted to calculate TMB (tumor mutational burden) for TCGA breast cancer samples. But I see that mutational load is available here Mutation Load - mutation-load-updated.txt for PAN CANCER data. So, From here I took the mutational load for TCGA BRCA data and it looks like below:
Cohort Patient_ID Tumor_Sample_ID Silent per Mb Non-silent per Mb
BRCA TCGA-A2-A0CO TCGA-A2-A0CO-01 0 0
BRCA TCGA-A2-A3KC TCGA-A2-A3KC-01 0 0
BRCA TCGA-A2-A3XS TCGA-A2-A3XS-01 0 0
BRCA TCGA-A2-A3XY TCGA-A2-A3XY-01 0 0
BRCA TCGA-A2-A4RW TCGA-A2-A4RW-01 0 0
BRCA TCGA-A2-A4RX TCGA-A2-A4RX-01 0 0
BRCA TCGA-A2-A4RY TCGA-A2-A4RY-01 0 0
BRCA TCGA-A2-A4S0 TCGA-A2-A4S0-01 0 0
BRCA TCGA-A7-A13H TCGA-A7-A13H-01 0 0
BRCA TCGA-A7-A3IY TCGA-A7-A3IY-01 0 0
BRCA TCGA-EW-A1P1 TCGA-EW-A1P1-01 0.028660581 0.028660581
BRCA TCGA-PL-A8LY TCGA-PL-A8LY-01 0.021965099 0.021965099
BRCA TCGA-A2-A25F TCGA-A2-A25F-01 0.029637804 0.059275609
BRCA TCGA-LL-A440 TCGA-LL-A440-01 0 0.045303438
BRCA TCGA-AO-A03U TCGA-AO-A03U-01 0.086896254 0.115861671
BRCA TCGA-A2-A0EP TCGA-A2-A0EP-01 0.021402315 0.107011574
BRCA TCGA-A2-A3XW TCGA-A2-A3XW-01 0.043349112 0.108372779
BRCA TCGA-OL-A5RY TCGA-OL-A5RY-01 0.023244128 0
BRCA TCGA-AR-A2LQ TCGA-AR-A2LQ-01 0.058017267 0.203060434
BRCA TCGA-OL-A5RV TCGA-OL-A5RV-01 0.045425394 0.158988878
BRCA TCGA-A2-A0ES TCGA-A2-A0ES-01 0.135797917 0.217276667
Using this information how can I divide samples into High TMB and Low TMB? Is there a cutoff? Any help is appreciated. thanq.