Hi, I uploaded my own gene predictions via a genbank file to RAST (preview of the genbank file below). RAST changed every single gene except for some RNA genes to the forward direction.
When I used RAST without my gene predictions and uploaded only the assembly in fasta format, genes were correctly oriented.
Question: how would I alter my genbank file so that RAST respects the directionality of each gene?
LOCUS scaffold3/1 168998 bp dna linear BCT DEFINITION XXXXX, unfinished sequence, whole genome shotgun sequence ACCESSION unknown KEYWORDS WGS. SOURCE Vibrio cholerae ORGANISM Vibrio cholerae Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. FEATURES Location/Qualifiers gene complement(942..259) /locus_tag="2011EL-1144H_draft_0312" CDS complement(942..259) /locus_tag="2011EL-1144H_draft_0312" /evidence=predicted /translation="[removed for brevity here in biostar]" /transl_table=11 /note="Predictors: gmhmmp, Glimmer3, BLAST"